Single-molecule tautomerization tracking through space- and time-resolved fluorescence spectroscopy

Time Resolved Single Molecule Spectroscopy

A new method based on the calculation of autocorrelation functions for spectra measured at a high acquisition rate is developed to study spectral dynamics of single molecules. The technique allows for spectroscopy with time resolutions down to the luminescence lifetime. The method is used to study spectral diffusion in two-photon excitation spectra of diphenyloctatetraene molecules doped in an ...

Time-resolved fluorescence spectroscopy

A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy.

We have recently developed a wide-field photon-counting detector having high-temporal and high-spatial resolutions and capable of high-throughput (the H33D detector). Its design is based on a 25 mm diameter multi-alkali photocathode producing one photo electron per detected photon, which are then multiplied up to 107 times by a 3-microchannel plate stack. The resulting electron cloud is proximi...

Single-molecule fluorescence spectroscopy and imaging

Observability of individual fluorescent molecules under a microscope has many applications in biology.1, 2 In particular, by grafting fluorophores to biomolecules, they can be tracked in real time in live cells for monitoring of their conformational changes and interactions with other molecules. In other words, singlemolecule imaging allows scientists to read the textbook of life pretty much as...

Time-Resolved Fluorescence Spectroscopy with LabView

1.1 Fluorescence phenomenon The absorption and subsequent emission of light by organic and inorganic specimens is typically the result of physical phenomena known as luminescence, which occurs at electronically excited states. Luminescence is formally divided into two categories: fluorescence and phosphorescence, depending on the nature of the excited state. Fluorescence occurs when a photon ex...